In this project, the preliminary work for a study of nuclear import of HIV-1 in macrophages was conducted. RNA inhibition was used to prevent expression of karyopherins a1, a2, b2, and RCC1. siRNA techniques were tested and refined for each of the samples on HeLa cells by using real time RT-PCR to establish that mRNA inhibition occurred and performing Western blots to ascertain that specific cellular protein titers were lowered.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Norgard, Elizabeth Anne

Publisher

• Worcester Polytechnic Institute

Identifier

• 03B007M

Advisor

• Adams, David S.

Year

• 2004

Sponsor

• University of Massachusetts Medical School

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright