Ependymin Immunoreactivity in Limulus polyphemusPublic
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Ependymin is a neurotrophic factor found during certain cellular events related to neuronal development, cellular regeneration, and memory consolidation. Since its discovery in goldfish in 1976, ependymin has been partially sequenced and shows notable conservation through many different species. This allowed for the development of an 8 aa antibody against ependymin which can be used to identify the protein's presence in various tissues. Using immunohistological techniques we have attempted to determine the location of ependymin in several stages of Limulus development. Our preliminary fluorescent staining of whole-mount crabs indicates a strong presence of ependymin throughout the central nervous system in the 1st, 3rd and 4th juvenile instar stages.
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