ART2 is a glycosylphosphatidylinositol-linked protein found on the surface of certain T lymphocytes, NK cells and IELs. ART2+ cell transfusion prevents insulitis in BBDP (diabetes prone) rats, while ART2+ cell depletion causes insulitis in BBDR (diabetes resistant) rats. Both strains serve as models for Type 1 Diabetes. During this project, a recombinant ART2.2 fusion construct designed for solubility and affinity purification was subcloned into an expression vector. Bacterial transformation resulted in expression of ART2.2 b protein intended to facilitate further investigation.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Chevrier, Lauren May

Publisher

• Worcester Polytechnic Institute

Identifier

• 04D163M

Advisor

• Adams, David S.

Year

• 2004

Sponsor

• University of Massachusetts Medical School

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

License

• All rights reserved