Chloride dependent K cotransport channel in human erythrocytes was investigated, in attempts to further elucidate the mechanism of control in regulatory volume decrease activated by Ca and propranolol. The effects of a phosphotyrosine phosphatase inhibitor, sodium orthovanadate and the protein kinase inhibitors, staurosporine and chelerythrine chloride were tested. Tests were conducted by mixing the inhibitor with washed erythrocytes, and buffered salline solution. A specialized computer program and apparatus measured the K efflux. Sodium orthovanadate inhibited K efflux at 1mM and 5mM; effects at higher concentrations were obscured by vanadate induced flux. Staurosporine and chelerythrine also inhibited K efflux. It was concluded that there is a protein kinase, possibly PKC, in the Ca/propranolol pathway, and a phosphotyrosine further downstream.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Surka, Susanne Anna

Publisher

• Worcester Polytechnic Institute

Identifier

• 02D291M

Advisor

• Hobey, William D.

Year

• 2002

Date created

• 2002-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright