Production of a NGF-TV A fusion protein for the potential targeting of an ALV-pseudotyped lentivirus to neuronal cellsPublic
Downloadable Contentopen in viewer
The purpose of this project was to develop a strategy based on guided adaptors for targeted entry (GATEs) technology to allow the targeted transduction of cell types of interest. In this project, the GATEs system was utilized in conjunction with ALV-pseudotyped lentivirus to target gene delivery to neurons by fusing the ligand nerve growth factor (NGF) to the N-terminus of the TVA receptor (for Avian Leukosis Virus subgroup A). The primary focus of this project was to determine if the fusion of NGF to the N-terminus of TVA results in a viable TVA receptor, since there is no precedence in the literature for the activity of TVA N-terminal fusion. The results indicate that the fusion of NGF to the N-terminus of TVA result in a viable TVA receptor since viral entry did occur.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Date created
- Resource type
- Rights statement
- In Collection:
|Thumbnail||Title||Visibility||Embargo Release Date||Actions|
Permanent link to this page: https://digital.wpi.edu/show/1c18dj81k