The purpose of this project was to design, construct and express a synthetic gene encoding a selected antimicrobial peptide. Based on the amino acid sequence of the protein, oligonucleotides corresponding to the gene coding region were designed and optimized according to the codon bias of Pichia pastoris, the yeast expression system. An archival plasmid containing the correct coding sequence of the gene was made but the construction of the expression vector and subsequent expression in P. pastoris is ongoing.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Michelon, Melissa Ann
• Borrelli, Alexander Peter

Publisher

• Worcester Polytechnic Institute

Identifier

• 01D308M

Advisor

• Bagshaw, Joseph C.

Year

• 2001

Date created

• 2001-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright