In this report, a more efficient technique to produce mAbs from Chinese Hamster Ovary cells in fed-batch systems is presented. A feed-forward control mechanism was created with BioXpert. The results are not conclusive due to lack of replicates to demonstrate reproducibility. If they are correct, it may be concluded that feed-forward control system was successfully implemented on the fed-batch bioreactors via BioXpert. Titer results from BioXpert experiments yielded higher results than manual feed-forward and similar to previous feedback experiments. For future work, it is recommended to repeat BioXpert feed-forward experiment with two or more replicates maintaining same cell line and using more frequent feeding schedule by keeping total daily feeding amount the same.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Altin Yaman, Ceren

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042408-160443

Advisor

• DiBiasio, David

Year

• 2008

Sponsor

• Abbott Bioresearch Laboratories

Date created

• 2008-04-24

Resource type

• Major Qualifying Project

Major

• Chemical Engineering

Rights statement

• In Copyright