Genome-Wide Short Hairpin RNA Screen for Senescence Genes
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open in viewerSmurf2 is an E3 ligase previously identified by our laboratory to be up-regulated by telomere shortening in senescent cells, and is sufficient to induce senescence in early passage human cells. Both the p53 and pRB senescence pathways are utilized by Smurf2 to induce senescence, but the precise mechanism of Smurf2-induced senescence remains unknown. The purpose of this project was to identify genes downstream of Smurf2 in the senescence pathways using a genome-wide short hairpin RNA (shRNA) screen in human fibroblasts. Using this strategy, I have identified 13 potential modulators of Smurf2-induced senescence, including AHCYL1 and CDC42BPA. Preliminary analysis indicates that AHCYL1 regulates senescence via the Smurf2-p53 pathway.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042909-125128
- Advisor
- Year
- 2009
- Sponsor
- Date created
- 2009-04-29
- Resource type
- Major
- Rights statement
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