The primary objective of this study was to determine the effects of CDC-like kinase 1 (CLK1) on differentiation of a telogen skin stem cell line as a follow-up study to Dr. Lyle's previous work on the differentiation of hair follicle cells. CLK1 knockdown strains were created using shRNA, then differentiated via several induction pathways. Cells lacking CLK1 differentiated along the sebocyte differentiation pathway similarly to the controls, but displayed no epidermal differentiation. These results suggest that CLK1 is necessary for epidermal differentiation, but plays no role in the differentiation of sebocytes.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Maurer, Jennifer Marie
• Duffty, Shannon Margaret

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042610-173218

Advisor

• Adams, David S.

Year

• 2010

Sponsor

• UMass Medical School

Date created

• 2010-04-26

Resource type

• Major Qualifying Project

Major

• Biochemistry
• Biology & Biotechnology

Rights statement

• In Copyright

License

• All rights reserved