Ependymin Immunoreactivity in Limulus polyphemus Public
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Ependymin (EPN) is a neurotrophic factor discovered in memory consolidation in goldfish. Sections of the EPN gene sequences have since been found in many other species, including the horseshoe crab. Previous research suggests that EPN may be instrumental in nerve growth. Using a new antibody against the bioactive 8 aa fragment of EPN, we have shown that EPN is present in the central and peripheral nervous system of developing horseshoe crabs. It was evident in the interstices between axons of regenerating limbs. HRP staining and fluorescence microscopy were used to observe EPN immunoreactivity. TEM and Immunogold staining were also employed to further support our results from fluorescence and light microscopy techniques and give us a more detailed view on EPN's mechanism of action.
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