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Evaluation of immunogold assays for amino acid neurotransmitters

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Agarose, gelatin, and PGA were assayed as potential matrices for adsorbing test solutions of amino acids, and gelatin was found to be the best candidate. Gelatin containing aspartate glutamate, or GABA was embedded in resin for ultrathin sectioning. Sections were treated with primary antibodies against amino acids and secondary gold-conjugated antibodies against the primaries, for examination by electron microscopy. The technique proved the reliability and specificity of antibodies against neurotransmitter candidates for applications in immunochemical studies using Transmission Electron Microscopy.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • 02D295M
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  • 2002
Date created
  • 2002-01-01
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