Translocation of Hepatocellular Mrp2 to the Canalicular Membrane Via Activation of PKC-delta by cAMPPublic
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Mrp2 is an organic anion and bile salt export pump that must be translocated from the cytoplasm to the hepatocyte tubule canalicular membrane to transport its substrate. Although cAMP has been shown to induce both Mrp2 translocation and PKC-delta activation, the interdependency of those two events has not been established. Hepatocytes were treated with a cAMP analogue and/or bistratene A (a known activator of PKC-delta) at varying concentrations, followed by biotinylation of cell surface protein, preparation of cell lysates, immunoprecipitation by streptavidin, and western analysis of Mrp2 levels. Like cAMP, bistratene A also induced Mrp2 translocation, and the combined effects were not additive, indicating that cAMP translocates Mrp2 by activating PKC-delta.
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