The SNARE complex, involved in vesicular trafficking and exocytosis, is composed of proteins in the vesicular membrane (V-SNAREs) that intertwine with proteins of the target membrane (T-SNAREs). Our results show that modified bovine neurosecretory granules (NSG) were able to spontaneously fuse with a planar lipid membrane, containing the t-SNARE syntaxin 1A. This provides evidence that syntaxin alone is able to form a functional fusion complex. This project is another step towards producing an artificial synapse to study possible regulatory components affecting vesicle fusion.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• McNally, James Matthew

Publisher

• Worcester Polytechnic Institute

Identifier

• 00D055M

Advisor

• Adams, David S.

Year

• 2000

Sponsor

• UMass Medical School

Date created

• 2000-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright