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The Role of Ependymin in Horseshoe Crab Limb Regeneration Public

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Immunohistology and a partial DNA sequence confirm the presence of the vertebrate neurotrophic factor ependymin in the horseshoe crab. We hypothesized that ependymin concentrates at sites of limb regeneration. We treated regenerating limb stumps prepared for light and electron microscopy with rabbit anti-ependymin (controls: normal rabbit serum) and goat anti-rabbit reporter antibodies. Ependymin is present at wound sites, but also diffusely present in limbs freshly removed from intact controls. Ependymin is likely necessary for normal growth as well as regeneration.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-042605-231549
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  • 2005
Date created
  • 2005-04-26
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