Characterizing the DNA-Binding Site Specificities of Cis2His2 Zinc FingersPublic
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The ability to modularly assemble Zinc Finger Proteins (ZFPs) as well as the wide variety of DNA sequences they can recognize, make ZFPs an ideal framework to design novel DNA-binding proteins. However, due to the complexity of the interactions between residues in the ZF recognition helix and the DNA-binding site there is currently no comprehensive recognition code that would allow for the accurate prediction of the DNA ZFP binding motifs or the design of novel ZFPs for a desired target site. Through the analysis of the DNA-binding site specificities of 98 ZFP clones, determined through a bacterial one-hybrid selection system, a predictive model was created that can accurately predict the binding site motifs of novel ZFPs.
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