Characterization and Use of Replication Competent HIV-1 Genomes Carrying Fluorescent Reporter GenesPublic
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HIV envelop genes specify which types of host cells the virus can infect. HIV clones carrying five different patient envelopes and two kinds of fluorescent reporter genes were constructed to develop tools for investigating viral tropisms. The data demonstrated that the new constructs showed the expected macrophage tropic or non-macrophage tropic phenotype and were detectable by FACS analysis. In future experiments, these clones will be extremely useful in evaluating whether highly macrophage-tropic envelopes confer a broader tropism among CD4-positive T-cell populations in blood lymphocyte cultures, and whether they out compete viral clones carrying non-macrophage tropic envelopes.
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