The purpose of this MQP was to clone and express the gene for the Salmonella secreted virulence protease, SipB, as a first step in developing a sensitive fluorescent peptide substrate assay for rapidly detecting Salmonella contamination. A test for such a protease would be specific for virulent Salmonella, not dead or latent bacteria. The SipB gene was amplified by PCR from Salmonella genomic DNA, cloned into an expression plasmid, and verified by sequence analysis. Immunoblots verified the presence of the expressed protein E. coli lysates. Induced cells showed more SipB in the insoluble protein fraction versus the soluble.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Wright, Melissa Ann

Publisher

• Worcester Polytechnic Institute

Identifier

• 00D027M

Advisor

• Adams, David S.

Year

• 2000

Sponsor

• Expressive Constructs, Inc

Date created

• 2000-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright