The DIG-1 protein in Caenorhabditis elegans is involved in neurological development and may be a proteoglycan. Experiments including immunoblotting, western analysis, acid proteolysis and ammonium precipitation were conducted to examine the protein. Immunoblotting showed that antibodies could be used to recognize the protein's c-terminus, which is missing in the n1480 mutant. Western analysis revealed a fragment that might be useful in determining the structure of DIG-1. If this is the expected fragment it should contain the glycosaminoglycans common in proteoglycans.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Reinsch, Joseph Edward

Publisher

• Worcester Polytechnic Institute

Identifier

• 04B004M

Advisor

• Ryder, Elizabeth F.
• Rulfs, Jill

Year

• 2004

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright