This project was to search for a DNA binding protein, LSF, in the switch region upstream of the gene encoding heavy chain 3 (S3). LSF was previously shown to bind the switch regions of the heavy chain and [mu] genes. LSF has been shown to bind DNA and may regulate class switching. Class switch recombination is an altering of the somatic cell DNA to produce antibodies that recognize the same antigen, but have different effects within the immune system. Using a radiolabeled oligonucleotide of S3 in vitro binding of a protein was observed by EMSA. Further competition experiments supported the hypothesis that LSF bind to S3.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Tongue, Philip Archer

Publisher

• Worcester Polytechnic Institute

Identifier

• 99D151M

Advisor

• Adams, David S.

Year

• 1999

Sponsor

• University of Massachusetts Medical School, Worcester, MA

Date created

• 1999-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

License

• All rights reserved