Turnip crinkle virus protein p8 has been shown to cooperatively bind RNA in vitro. Current techniques for analyzing this protein/RNA interaction have inherent limitations, including time required to perform the assay and lack of quantitative data. Capillary electrophoresis (CE) was evaluated as a potential platform to overcome these shortcomings, ideally providing both rapid analysis and quantifiable data. Due to several complicating factors, a CE assay is shown to be too difficult to perform and analyze to be of practical value.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Harang, Richard Edward

Publisher

• Worcester Polytechnic Institute

Identifier

• 00D145M

Advisor

• Wobbe, Kristin K.

Year

• 2000

Date created

• 2000-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright