TY - RPRT A1 - Popinchalk, Samuel Paul AB - The murine antibody, 125-2H, is a potent neutralizer of interleukin (IL-18), which is associated with inflammation. Because of its murine origins it is not a clinical candidate, but its IL-18 neutralizing potency is the standard to which human monoclonal antibodies to IL-18 are held. To determine the amino acid residues most essential to its interaction with IL-18, alanine scan mutagenesis was performed on the third complementarity-determining regions (CDR3) of the light and heavy chains. Following testing in a KG-1 assay for IL-18 neutralizing ability, it was determined that the alanine substitutions at four CDR3 amino acid residues of the heavy chain are most essential for IL-18 binding. DA - 2002/01/01 DB - Digital WPI DP - Worcester Polytechnic Institute ID - 02D526M L1 - https://digital.wpi.edu/show/kk91fp384 LK - https://digital.wpi.edu/ PB - Worcester Polytechnic Institute PY - 2002 T1 - Alanine scan mutagenesis in CDR3 of 125-2H UR - https://digital.wpi.edu/show/c821gn71k ER -