In this report, the biophysical parameters of metastatic MDA-MB-231 and non-carcinoma MCF10a mammary cell lines are collected on 2D hydrogels and in confinement, created in vitro by sandwiching cells between two planar hydrogels. Traction force microscopy (TFM) was used to obtain parameters such as traction forces and strain energy, whereas image analysis was used to obtain morphological parameters. Looking at these parameters in tandem can provide insight into how the phenotype or cytoskeletal structure may change in and out of confinement. This paper can provide a better understanding of the metastatic cascade, and thus lay the foundations for future treatments and therapies of cancer.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Miguel, Mason

Subject

• Health

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050323-153252
• 108526

Keyword

• Traction Force Microscopy
• Metastasis
• Cancer Cell Mechanics
• Biophysics

Advisor

• Wen, Qi

Year

• 2023

Date created

• 2023-05-03

Resource type

• Major Qualifying Project

Major

• Physics

Source

• E-project-050323-153252

Rights statement

• In Copyright

Last modified

• 2023-06-13