The Kekkon family, represents a subfamily of LIGs in Drosophila melanogaster. Previous experiments suggested that Kek2 and Kek6 exhibit preferential homo- and heterophilic binding and could represent novel cell adhesion molecules. To further investigate this, these and possibly interactions among other Kek family members were pursued in cell aggregation and extracellular protein-protein (ePPI) interaction assays. While the cell aggregation assay was unable to be completed, no interactions between any Kek family members were detected in the the ePPI assay. Suggestions to overcome experimental pitfalls include: for cell adhesion - generating stable cell lines or adopt to a cell surface binding assay; for ePPI – confirm presence of protein tag/size via Western blot and generating monomeric protein tags.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Allen, Hannah

Publisher

• Worcester Polytechnic Institute

Identifier

• 107086
• E-project-042923-215233

Advisor

• Duffy, Joseph B.

Year

• 2023

Date created

• 2023-04-29

Resource type

• Major Qualifying Project

Major

• Biotechnology

Source

• E-project-042923-215233

Rights statement

• In Copyright

Ultima modifica

• 2023-06-20