A Method for the Tubular Self-Assembly of Cells to Create Tissue Engineered Blood VesselsPublic
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The purpose of the project was to develop a method to induce the self-assembly of cells into a tubular construct. To do this, we utilized the concept of centrifugal force. Cells were inserted into a non-adhesive hollow polycarbonate mandrel. The mandrel was spun for 15 minutes, forcing the cells to distribute themselves against the inner wall of the mandrel. After spinning, the mandrel was placed in a bioreactor and incubated for 3 days. During incubation, the cells aggregated and contracted off the mandrel, forming a tubular construct. This cellular tube was then removed from inside the mandrel using forceps. Histology was used to examine tissue structure and distribution of cells. The harvested tubes exhibited dense aggregates of cells indicative of a cohesive tissue.
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