Oligosaccharyl Transferase (OST) N-glycosylates nascent polypeptides as they are being translocated into the endoplasmic reticulum (ER). Mammalian OST contains eight subunits, of which STT3 is believed to be the catalytic one. There are two isoforms of STT3, STT3-A and STT3-B, which have different kinetic and substrate recognition properties. Interestingly, it was found that both isoforms are present in cells from a variety of tissues. However, it is unclear if both are needed for proper functioning of OST. RNAi will be used to knockdown expression of one of the isoforms in HEK293 cells to measure differing effects in N-glycosylation of proteins.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Hands, Michael David

Contributors

• Gilmore, Reid

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042805-153626

Advisor

• Adams, David S.

Year

• 2005

Sponsor

• Umass Medical Center

Date created

• 2005-04-28

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

License

• All rights reserved

Last modified

• 2021-09-11