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Ribozyme viral infection of Hct116 cells Public

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Ribozymes are being researched because of the clues they provide about evolutionary history, and the possibilities they provide as therapy against many viruses and certain types of cancer. The purpose of this project was to create a mutagenesis gene discovery system for identifying genes that block apoptosis using a randomized hammerhead ribozyme library. There were three main steps to this project: creating a randomized hammerhead ribozyme library, packaging the library in a retroviral vector, and infecting Hctl 16 cells for mutagenesis. The ribozyme library was proven to be correctly constructed and randomized, as well as capable of being packaged and able to infect cells.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • 03B031M
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  • 2004
Date created
  • 2004-01-14
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