The goal of this MQP was to develop the necessary methodology to initiate the characterization of six heavy metal transport ATPases found in Arabidopsis thaliana. Growth of Arabidopsis thaliana in an MS salt media, optimal subtoxic metal ion level (50 uM CuSO4, 50 uM ZnSO4, and 10 uM CdCl2), And RNA extraction were standardized. Two probes were designed for two genes (AAD29115, AAD12041). A Northern blot of the target genes was performed.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Dupont, Joseph

Publisher

• Worcester Polytechnic Institute

Identifier

• 01D273M

Advisor

• Argüello, José M.

Year

• 2001

Date created

• 2001-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright