Cellular transfection, or the delivery of nucleic acids into living cells, has applications ranging from personalized medicines to biomanufacturing. Currently, a variety of transfection techniques exist, however these methods are limited in their applications, often restricted to certain cell types and cargos. Additionally, transfection methods currently employed for applications in cell-based therapies are expensive and inefficient, increasing the cost of life-saving medicines like CAR-T therapy. The development of an efficient transfection device that can be applied across cell types or cargo would potentially increase transfection capabilities. In this study, a simple and easy-to-use microfluidic transfection device was designed and tested for transfection of dextran sulphate, DNA, and proteins into mouse 3T3 cells. Our study demonstrates the proof-of-principle for using microfluidics for cell transfection in vitro.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Nguyen, Benjamin
• Defreitas, Steven
• Frick, Emily
• Panza, Gianluca

Subject

• Well-Being
• Health
• Innovation
• Industry

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042723-110014
• 105951

Keyword

• Intracellular Delivery
• Primary Cells
• Microfluidics
• CAR-T Therapy
• Cell Membrane
• Transfection

Advisor

• Ambady, Sakthikumar
• Gnanaskandan, Aswin
• Sabuncu, Ahmet Can

Year

• 2023

UN Sustainable Development Goals

• 3 - Good Health and Well-being
• 9 - Industry, Innovation and Infrastructure
• 10 - Reducing Inequality

Date created

• 2023-04-27

Resource type

• Major Qualifying Project

Major

• Mechanical Engineering
• Biomedical Engineering

Source

• E-project-042723-110014

Rights statement

• In Copyright

Last modified

• 2023-06-21