Recombinant Cloning and Expression of Viral Proteins From West Nile and Dengue VirusesPublic
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Recent studies with Dengue (DEN) and West Nile (WN) viruses have focused on structural analyses, and host cellular and immunological responses to viral infection. In this project, various DEN and WN proteins were expressed in E. coli using recombinant expression plasmids. Expression was verified by RT-PCR and immunoblots. The expressed proteins will be used in the future to test T-cell reactivities against viral proteins.
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