Two monosaccharide labeling agents, 1-phenyl-3-methyl-5-pyrazolone (PMP), and 4-(3-Methyl-5-oxo-2-pyrazolin-1-yl) Benzoic Acid (PMPA), were investigated to determine monosaccharide content in palm oil mill effluent waste (POME). Methods published in the literature were optimized to increase efficiency of monosaccharide detection to suit industry needs. The results from PMP derivatization, using pH 8.0 PBS for dilution and microwave irradiation suggest this is the most efficient derivatization method tested in this project. Monosaccharides detected in POME samples were mannose, glucose and xylose.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Ramapriya, Girija Champaka

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-121307-113327

Advisor

• Rulfs, Jill

Year

• 2007

Center

• MIT Lincoln Laboratory-Lexington, Massachusetts Project Center - MQP

Sponsor

• Massachusetts Institute of Technology Biomaterials

Date created

• 2007-12-13

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

License

• All rights reserved