Optimization of Monosaccharide LabelingPublic
Downloadable Contentopen in viewer
Two monosaccharide labeling agents, 1-phenyl-3-methyl-5-pyrazolone (PMP), and 4-(3-Methyl-5-oxo-2-pyrazolin-1-yl) Benzoic Acid (PMPA), were investigated to determine monosaccharide content in palm oil mill effluent waste (POME). Methods published in the literature were optimized to increase efficiency of monosaccharide detection to suit industry needs. The results from PMP derivatization, using pH 8.0 PBS for dilution and microwave irradiation suggest this is the most efficient derivatization method tested in this project. Monosaccharides detected in POME samples were mannose, glucose and xylose.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Date created
- Resource type
- Rights statement
- In Collection:
Permanent link to this page: https://digital.wpi.edu/show/k35695762