Analysis of QK-1 Binding to mRNA Targets Implicated in the Schizophrenia PathwayPublic
Downloadable Contentopen in viewer
Down regulation of QK-1 correlates with reduced levels of SFRS5,hnRNP A1, NCAM, hnRNP D, and SRRM 1RNA. QK-1 is an RNA binding protein that may regulate the localization and stability of these mRNA transcripts. To test if QK-1 can bind to these candidate targets, quantitative binding experiments were performed using fluorescence polarization, competition fluorescence polarization, and gel mobility shift assays. The data show that QK-1 binds with the highest affinity to hnRNP A1, NCAM and SRRM 1 transcripts, consistent with direct role for this protein in the regulation of these mRNAs. Interestingly, like QK-1, NCAM is also suspected to play a role in schizophrenia. The data show that QK-1 binds tightly to NCAM RNA, establishing a new link between these two schizophrenia candidates.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Date created
- Resource type
- Rights statement
- In Collection:
Permanent link to this page: https://digital.wpi.edu/show/ks65hf03k