Histone Nuclear Factor-P (HiNF-P) is the final link in a CyclinE/CDK2, p220NPAT pathway to cell cycle-dependent transcription of histone H4. In this project, mutations were introduced to HiNF-P to study the biological effects of the P-Specific Conserved Region (PSCR), a highly conserved region whose specific biological activity is unknown. Assays were carried out to quantify expression (Western), subcellular localization (immunofluorescence), DNA binding (EMSA), and H4 promoter activity (luciferase). The data strongly suggest that the first twenty amino acids of the PSCR are essential for DNA binding.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Buck, Timothy F.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-121506-130719

Advisor

• Adams, David S.

Year

• 2006

Date created

• 2006-01-10

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

License

• All rights reserved