The Human Papillomavirus E2 protein is involved in multiple processes including transcriptional regulation of viral early genes and the stable maintenance of viral genomes. E2 has been shown to associate with mitotic chromosomes. However, the mechanism of this interaction. is unknown. Previously, E2 was shown to bind to protein CHLr1, a DNA helicase required for high fidelity chromosome maintenance during mitotic segregation. In this project, E2 mutants that do not bind to CHLr1 were isolated using the yeast two-hybrid system. Analysis of such mutants will help in determining the functional significance of the interaction.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Bean, Angela Marie

Publisher

• Worcester Polytechnic Institute

Identifier

• 04D278M

Advisor

• Adams, David S.

Year

• 2004

Sponsor

• UMass Medical School

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright