Developing a FRET-based Activation Indicator in Ste2p, a Yeast GPCR
公开Of great interest to the pharmaceutical market, GPCR behavior is still not well understood. The interplay between ligand binding, receptor activation, and the resulting cellular response is not well categorized. Current assays to measure activation are indirect and rely on downstream activation of reporter genes. Successful in other GPCRs, a FRET-based direct indicator of activation was developed in Ste2p. Identification of a successful clone will allow further studies of GPCR behaviors, such as fractional occupancy. Pharmaceutical dosing of GPCR-targeted medicines relies on understanding the dynamic relationship of binding, activation, and output that impacts receptor behavior.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- 贡献者
- Publisher
- Identifier
- E-project-042618-090854
- Advisor
- Year
- 2018
- Sponsor
- Date created
- 2018-04-26
- Resource type
- Major
- Rights statement
关系
- 属于 Collection:
项目
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FRET-based_Activation_Indicator_in_Ste2p_V.Johnson_2018.pdf | 公开 | 下载 |
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