%0 Student Work %T WIF-B cell line analysis %A Usechak, Paul Julian %D 2000-01-01 %8 2021-09-17 %I Worcester Polytechnic Institute %R https://digital.wpi.edu/show/n870zv059 %X The aim of this project was to determine if the WIF-B cell line, a mouse hepatoma-human fibroblast hybrid, could be used as a model system for bile induced hepatocyte apoptosis as an alternative to primary cell culture. This is of particular interest because bile acid induced hepatocyte apoptosis accompanies cholestatic liver disease as a result of impaired bile flow. The results of this study revealed that WIF-B cells do not undergo apoptosis following exposure to bile acids. This is most likely due to the lack of significant membrane transport of bile acids in this cell line. %9 Major Qualifying Project %~ Digital WPI %W WPI