The aim of this project was to determine if the WIF-B cell line, a mouse hepatoma-human fibroblast hybrid, could be used as a model system for bile induced hepatocyte apoptosis as an alternative to primary cell culture. This is of particular interest because bile acid induced hepatocyte apoptosis accompanies cholestatic liver disease as a result of impaired bile flow. The results of this study revealed that WIF-B cells do not undergo apoptosis following exposure to bile acids. This is most likely due to the lack of significant membrane transport of bile acids in this cell line.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Usechak, Paul Julian

Publisher

• Worcester Polytechnic Institute

Identifier

• 00B026M

Advisor

• Rulfs, Jill

Year

• 2000

Date created

• 2000-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

License

• All rights reserved