Student Work

Expression of dig-1 in Caenorhabditis elegans

Public

The dig-1 gene in Caenorhabditis elegans encodes an adhesion molecule in the immunoglobulin superfamily that is important to nervous system development. To determine time and location of DIG-1 protein expression, wild type and mutant gravid adults were embedded and sectioned. Sections were held to glass slides using heat and stained with two antibodies specific to DIG-1. Mutant strain n1480, which is missing the C-terminus portion of the protein which contained the CON-3 binding site, was used to compare the specificity of the anti-CON-3 against wild type. Initial results suggest that DIG-1 is expressed only in the developing embryos of C. elegans. The specificity of the anti-CON-3 was shown in that it showed little if no binding in the mutant n1480, which expresses a truncated DIG-1 protein and is hypothesized to not have the CON-3 binding site. Fixing, embedding, and molding techniques were also tested to determine efficiency of processes.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
Creator
Publisher
Identifier
  • 03D128M
Advisor
Year
  • 2003
Date created
  • 2003-01-01
Resource type
Major
Rights statement

Relations

In Collection:

Items

Items

Permanent link to this page: https://digital.wpi.edu/show/pk02cd63s