Student Work

Identification of proliferating cells in hard shell clams using anti-PCNA antibodies

Public

The hemocyte-producing tissue in hard shell clams (genus Mercenaria) is unknown. In this MQP, an antibody against Proliferating Cell Nuclear Antigen (PCNA) was used to identify areas of rapid cell division in the clam, with the goal of locating hematopoietic tissue. Anti-PCNA immunoreactivity was detected in tissues known to cycle rapidly (e.g. ovotestis and gut epithelium), and in some hemocytes in and around foci of inflammation. These findings indicate that mouse anti-PCNA recognizes the clam epitope. Furthermore, when triggered by an immune response, hematopoiesis in the hard clam may be occurring directly at the site of inflammation.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
Creator
Publisher
Identifier
  • 00B018M
Advisor
Year
  • 2000
Sponsor
Date created
  • 2000-01-01
Resource type
Major
Rights statement

Relations

In Collection:

Items

Items

Permanent link to this page: https://digital.wpi.edu/show/pk02cd72r