Effects of Silencing the Mental Retardation Gene Jarid1c on Neuronal Differentiation of Pluripotent P19 CellsPublic
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Mutations in the Jarid1c gene have been found to cause mental retardation but it is not clear exactly how this occurs. Jarid1c may play a role in neuronal differentiation in the embryonic brain. Jarid1c was silenced in P19 cells using the Lentivirus. Neuronal differentiation was induced in Jarid1c knockdown P19 cells and in control P19 cells. The effect of silencing Jarid1c was accessed by PCR. Fus and Gfra3 were found to be upregulated in the Jarid1c knockdown on Day 0. On Day 4, Ntrk1 was upregulated while Il1r1 and Il6 were downregulated in the Jarid1c knockdown cells. By Day 10, P19 cells were fully differentiated and there was no detectable difference. These results indicate that Jarid1c is involved in neuronal differentiation as well as in gene expression in pluripotent stem cells.
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