An effective antibody response is essential for immunity against influenza (Flu) virus infection and is the primary goal for Flu vaccines. In this study, codon optimized and wild type DNA vaccines expressing hemmaglutinin (HA) antigens of human flu viruses A/H1N1/NewCal/20/99 (H1 serotype) were compared. Expression levels of HA antigens were increased in 293T cells transiently transfected with codon optimized as compare to the wild type HA gene construct. The codon optimized vaccine was able to elicit a greater immune response in rabbits and mice as measured by ELISA testing of the temporal response and end antibody titers.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Parker, Christopher S

Contributors

• Shan Lu

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-010406-095229

Advisor

• Adams, David S.

Year

• 2006

Sponsor

• University of Massachusetts Medical School

Date created

• 2006-01-04

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

License

• All rights reserved