Comparison of Codon Optimized and Non-Optimized Influenza HA DNA VaccinesPublic
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An effective antibody response is essential for immunity against influenza (Flu) virus infection and is the primary goal for Flu vaccines. In this study, codon optimized and wild type DNA vaccines expressing hemmaglutinin (HA) antigens of human flu viruses A/H1N1/NewCal/20/99 (H1 serotype) were compared. Expression levels of HA antigens were increased in 293T cells transiently transfected with codon optimized as compare to the wild type HA gene construct. The codon optimized vaccine was able to elicit a greater immune response in rabbits and mice as measured by ELISA testing of the temporal response and end antibody titers.
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