Geldanamycin treatment of precision-cut liver slices increases aspartate aminotransferase (AST) levels in both the slices and corresponding media. The pathway that causes this enzyme increase was investigated through extensive literature research and it was found that increased levels of unbound heat shock protein 70 (hsp70) probably plays an important role. The study used quercetin, an inhibitor of hsp70, in an attempt to decrease the AST levels. However, the results were inconclusive and did not support nor disprove the discovered pathway.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Pursell, Bryan Michael.
• Cheng, Ting-Ting.
• Coughlin, Amy Kathrine.

Publisher

• Worcester Polytechnic Institute

Identifier

• 05D006M

Advisor

• Rulfs, Jill

Year

• 2005

Date created

• 2005-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright