CopA P1B-ATPase from A. fulgidus transports Cu/Ag. The Cysteines in the CPC sequence in H6 are involved in metal coordination. However, the mechanism of metal selectivity remains unknown. To elucidate their role in in metal transport, five conserved residues were mutated by site directed mutagenesis. Two mutants from H7 (Y682A, N683A) and one from H8 (M711A) are inactivated; these residues participate in metal coordination. Mutants N675A and P688A retain partial activity and metal affinity; these residues do not participate in metal coordination.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Kertesz, Tzipporah M.

Publisher

• Worcester Polytechnic Institute

Identifier

• 04D042M

Advisor

• Argüello, José M.

Year

• 2004

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright

License

• All rights reserved