Development of Spatial Optical Coherence Tomography Methods to Characterize Thin Biological SamplesPublic
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Obtaining thicknesses of micro-scale samples through noninvasive methods is crucial when dealing with fragile or biological samples. Optical Coherence Tomography (OCT) is an imaging technique which uses low coherence interferometry to create a layered stack of cross-sectional images without directly contacting the sample. An OCT setup was developed for our experimentation, was validated for measurement accuracy with samples of known thickness with an error of ±4.73%, and applied to characterize specific biological samples. Our OCT enables the observation of both, surfaces and internal structures of micro-scale samples.
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