The intent of this project was to determine optimal separation conditions for the use of affinity partitioning to separate human serum albumin from chicken egg whites. The two-phase PEG-DEX system was analyzed and the use of a PEG-palmitate affinity ligand was investigated. It was our goal to determine the system conditions that would lead to the highest possible recovery of HSA from the system. Also completed was a proposal for an industrial-scale separation process for the system conditions that were deemed optimal.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Cook, Bradley James

Publisher

• Worcester Polytechnic Institute

Identifier

• 04D266M

Advisor

• Clark, William M.

Year

• 2004

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Chemical Engineering

Rights statement

• In Copyright