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Design of a Co-culture System Using Collagen Microthreads to Facilitate Neovascularization Public

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Several co-culture systems involving fibroblasts and endothelial cells have been used to characterize angiogenesis. However, these models have not been translated into therapies for chronic non-healing wounds. We developed an approach that may facilitate in vivo neovascularization and serve as an in vitro tool to study angiogenic mechanisms. Self-assembled collagen microthreads populated with human dermal fibroblasts (HDFs) were extruded using a novel method prior to surface seeding of human umbilical vein endothelial cells (HUVECs). Fluorescence microscopy confirmed the presence of both cell types after 48 hours of co-culture. These results demonstrate that a viable co-culture system can be established on collagen microthreads with these seeding methods.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
Last modified
  • 02/02/2021
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  • E-project-043009-105252
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Year
  • 2009
Date created
  • 2009-04-30
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Permanent link to this page: https://digital.wpi.edu/show/s4655j14w