Several co-culture systems involving fibroblasts and endothelial cells have been used to characterize angiogenesis. However, these models have not been translated into therapies for chronic non-healing wounds. We developed an approach that may facilitate in vivo neovascularization and serve as an in vitro tool to study angiogenic mechanisms. Self-assembled collagen microthreads populated with human dermal fibroblasts (HDFs) were extruded using a novel method prior to surface seeding of human umbilical vein endothelial cells (HUVECs). Fluorescence microscopy confirmed the presence of both cell types after 48 hours of co-culture. These results demonstrate that a viable co-culture system can be established on collagen microthreads with these seeding methods.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Carey, Shawn P.
• Ellis, Elizabeth C.
• Hu, Jason Zicheng
• Charest, Jonathan Marc

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-043009-105252

Advisor

• Pins, George D
• Rolle, Marsha Whitney

Year

• 2009

Date created

• 2009-04-30

Resource type

• Major Qualifying Project

Major

• Chemical Engineering
• Biomedical Engineering

Rights statement

• In Copyright

License

• All rights reserved

Last modified

• 2021-02-02