Phospholipase C β1 (PLCβ1) is a protein involved in a signal transduction pathway that causes a cell to release Ca2+ into the cytoplasm as a result of G protein activation. Argonaute 2 (Ago2) is a multifunctional protein involved in RNA silencing leading to mRNA degradation and is incorporated into protein-mRNA aggregates called stress granules (SGs). Stress granules help cells survive stressful environmental conditions by protecting mRNA and proteins from degradation. Component 3 promoter of RISC (C3PO) is a member of the RNA induced silencing complex (RISC) that is composed of TRAX and translin subunits. PLCβ1 binds to both C3PO and Ago2 in the cytosol to inhibit RNAi activity and mediate stress granule aggregation, respectively. Here we show evidence of interaction between these proteins and we have implemented a procedure in which proteins could be purified to investigate the molecular details of their association.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Stillings, Sara

Publisher

• Worcester Polytechnic Institute

Identifier

• 65881
• E-project-042822-162430

Keyword

• Protein Purification
• C3PO
• TRAX
• Ago2
• PLCβ1
• RISC complex

Advisor

• Scarlata, Suzanne Frances

Year

• 2022

Date created

• 2022-04-28

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright