A Novel Technique for the Expression and Purification of HIV-1 VIF Co-factor APOBEC3GPublic
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HIV-1 has evolved to protect itself against the body's innate viral defense mechanisms. One such example of this phenomenon is illustrated by the viral protein vif, which is expressed in the later stages of infection. Vif binds the host cell protein Apobec3G, a cytidine deaminase which is ectopically expressed in non-permissive cell lines to allow for reverse transcription of the HIV-1 viral genome. Apobec3G has the ability to prevent HIV-1 replication by mutating the cDNA, so the goal of this research was to develop a novel technique for the expression and purification of Apobec3G. An expression and purification technique was developed, and the purified Apobec3G protein was shown to have the capacity for binding viral Vif in vitro.
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