Production of a putative mouse homolog for Protein AS160 and its use for antibody formulation for studying GLUT4 trafficking in adipocytesPublic
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GLUT4 trafficking is an integral process in insulin signaling. There are many different proteins involved, but a particular interesting protein is a substrate of Akt called AS160. AS160 was found to be insulin sensitive, being down-regulated in the cytosol, and up-regulated in low density microsomes. Working with Michael Czech at UMASS Medical Center, the focus of this MQP was to develop an antibody to mouse AS160 with a GST tag at the N-terminus. The antibody was then used with 3T3-L1 adipocytes as an in-vitro cell model to study the role of AS160 in GLUT4 trafficking.
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