GLUT4 trafficking is an integral process in insulin signaling. There are many different proteins involved, but a particular interesting protein is a substrate of Akt called AS160. AS160 was found to be insulin sensitive, being down-regulated in the cytosol, and up-regulated in low density microsomes. Working with Michael Czech at UMASS Medical Center, the focus of this MQP was to develop an antibody to mouse AS160 with a GST tag at the N-terminus. The antibody was then used with 3T3-L1 adipocytes as an in-vitro cell model to study the role of AS160 in GLUT4 trafficking.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Pleau, Cara Marie

Publisher

• Worcester Polytechnic Institute

Identifier

• 03D203M

Advisor

• Adams, David S.

Year

• 2003

Date created

• 2003-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright