Though Turnip Crinkle Virus (TCV) has been demonstrated to encode a suppressor of RNA silencing, no research has shown whether small interfering RNA's (siRNAs) specific to TCV can be isolated from infected Arabidopsis thaliana. In order to explore this, a protocol for isolating and probing siRNAs was developed using siRNA literature as a guide. This study also demonstrates that no WT-TCV specific siRNA produced in infected Col-0 or Di-17 A. thaliana strains and provides a groundwork for future siRNA related studies in this system.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Collins, Jacob Marz

Publisher

• Worcester Polytechnic Institute

Identifier

• 03D091M

Advisor

• Wobbe, Kristin K.

Year

• 2003

Date created

• 2003-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright