EFFECTS OF ALCOHOL ON THE LOCALIZATION OF INSERTLESS AND STREX, BK CHANNEL ALPHA SUBUNIT ISOFORMSPublic
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Alcohol affects a variety of physiological and cellular processes. Large-conductance calcium-activated (BK) potassium channels are one such target. Ethanol binds to these receptors to alter cellular distribution and hinder cellular repolarization. BK channel alpha subunits are sensitive to ethanol's effects, and recently were shown to exist as different isoforms, including "Insertless" and "STREX". This project used immunofluorescence microscopy to analyze cell membrane distribution of Insertless and STREX in transfected HEK cells with and without ethanol exposure. The data show that ethanol downregulates Insertless and causes receptor declustering, while the STREX isoform is largely resistant to ethanol's effects, and overall BK membrane intensity decreases with exposure to ethanol.
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